Comparison of the altimetric signal with in situ measurements in the Tropical Atlantic ocean

An intensive survey of XBT and surface salinity sampling was carried out during september/october 1988 to compare surface dynamic heigh anomalies and altimetric anomalies along a line close to a GEOSAT satellite track in the Tropical Atlantic ocean, 15°N-18°S. Hydrography and altimetry agree within 4 cm rms, except in the Southern part of the section (South of 5°S). In the Northern hemisphere, the correlation between the two data sets is about 0.80 and the two power spectra present the same energy level .... The hydrographic data show an unusual tongue of fresh water around 7°N that results in positive dynamic height anomalies of about 10 dyn cm. The GEOSAT data analysed during the same period also show these anomalies with a similar scale. (D'après résumé d'auteur

Geosat sea-level assimilation in a tropical Atlantic model using Kalman filter

We present preliminary results on Geosat altimetric data assimilation in a linear vertical mode model of the tropical Atlantic Ocean. The Kalman filter technique is used to assimilate altimetric data along one track at a time as the satellite over-flies the basin. Sensitivity and validation tests have been performed with simulated data. The results obtained with Geosat data are presented and compared on a monthly basis with objective analysis of altimetric data and oceanic general circulation model results

Comparative assimilation of Topex/poseidon and ERS altimetric data and of TAO temperature data in the tropical Pacific Ocean during 1994-1998, and the mean sea-surface height issue

International audienceFive years of Topex/Poseidon (T/P) and ERS sea level anomaly (SLA) data (1994–1998) are assimilated every 10 days into a primitive equation model of the tropical Pacific ocean. The data assimilation technique used here is a reduced-order Kalman filter derived from the Singular Evolutive Extended Kalman (SEEK) filter [J. Mar. Syst. 16(3–4) (1998) 323] with an error covariance matrix parameterised by a subset of multivariate 3D global empirical orthogonal functions (EOFs). The assimilation run is compared to the free run and to independent data from the TAO network. The impact of sea-surface height (SSH) assimilation on surface and subsurface temperature and currents is estimated in the equatorial band. In a second stage, temperature data from the TAO array are assimilated in the same conditions as in the first stage. The comparison between the results of the two assimilation experiments is made mainly with a view to gaining insights into the mean sea-surface height (MSSH) for the assimilation of altimeter data, and more generally, into the question of biases. XBT observations and TAO array data are then used to build a physically more consistent mean sea-surface height for assimilation of SLA data. Results from the assimilation of altimeter data referenced to this new MSSH show significant improvements

Synergistic activity of troxacitabine (Troxatyl™) and gemcitabine in pancreatic cancer

<p>Abstract</p> <p>Background</p> <p>Gemcitabine, a deoxycytidine nucleoside analog, is the current standard chemotherapy used as first-line treatment for patients with locally advanced or metastatic cancer of the pancreas, and extends life survival by 5.7 months. Advanced pancreatic cancer thus remains a highly unmet medical need and new therapeutic agents are required for this patient population. Troxacitabine (Troxatyl™) is the first unnatural L-nucleoside analog to show potent preclinical antitumor activity and is currently under clinical investigation. Troxacitabine was recently evaluated as a first-line therapy in 54 patients with advanced adenocarcinoma of the pancreas and gave comparable overall results to those reported with gemcitabine in recently published randomized trials.</p> <p>Methods</p> <p>The human pancreatic adenocarcinoma cell lines, AsPC-1, Capan-2, MIA PaCa-2 and Panc-1, were exposed to troxacitabine or gemcitabine alone or in combination, for 72 h, and the effects on cell growth were determined by electronic particle counting. Synergistic efficacy was determined by the isobologram and combination-index methods of Chou and Talalay. Mechanistic studies addressed incorporation of troxacitabine into DNA and intracellular levels of troxacitabine and gemcitabine metabolites. For <it>in vivo </it>studies, we evaluated the effect of both drugs, alone and in combination, on the growth of established human pancreatic (AsPC-1) tumors implanted subcutaneously in nude mice. Statistical analysis was calculated by a one-way ANOVA with Dunnett as a post-test and the two-tailed unpaired <it>t </it>test using GraphPad prism software.</p> <p>Results</p> <p>Synergy, evaluated using the CalcuSyn Software, was observed in all four cell-lines at multiple drug concentrations resulting in combination indices under 0.7 at Fa of 0.5 (50% reduction of cell growth). The effects of drug exposures on troxacitabine and gemcitabine nucleotide pools were analyzed, and although gemcitabine reduced phosphorylation of troxacitabine when cells were exposed at equal drug concentrations, there was no effect on phosphorylated pools at drug combinations that were synergistic. The amount of troxacitabine incorporated into DNA was also not affected by the presence of gemcitabine. <it>In vivo </it>testing against a human pancreatic (AsPC-1) xenograft mouse tumor model indicated that both drugs were more than additive at well-tolerated doses and schedule. The biological basis for this synergy is unclear as we did not observe changes in apoptosis, DNA repair, troxacitabine incorporation into DNA or troxacitabine metabolism in the presence of gemcitabine.</p> <p>Conclusion</p> <p>These data, together with phase I clinical data showing tolerability of both agents when combined, suggest combination therapy with troxacitabine and gemcitabine warrants further evaluation in advanced pancreatic cancer patients.</p

Spectral signatures of the tropical Pacific dynamics from model and altimetry: a focus on the meso-/submesoscale range

The processes that contribute to the flat sea surface height (SSH) wavenumber spectral slopes observed in the tropics by satellite altimetry are examined in the tropical Pacific. The tropical dynamics are first investigated with a 1∕12° global model. The equatorial region from 10°&thinsp;N to 10°&thinsp;S is dominated by tropical instability waves with a peak of energy at 1000&thinsp;km wavelength, strong anisotropy, and a cascade of energy from 600&thinsp;km down to smaller scales. The off-equatorial regions from 10 to 20° latitude are characterized by a narrower mesoscale range, typical of midlatitudes. In the tropics, the spectral taper window and segment lengths need to be adjusted to include these larger energetic scales. The equatorial and off-equatorial regions of the 1∕12° model have surface kinetic energy spectra consistent with quasi-geostrophic turbulence. The balanced component of the dynamics slightly flattens the EKE spectra, but modeled SSH wavenumber spectra maintain a steep slope that does not match the observed altimetric spectra. A second analysis is based on 1∕36° high-frequency regional simulations in the western tropical Pacific, with and without explicit tides, where we find a strong signature of internal waves and internal tides that act to increase the smaller-scale SSH spectral energy power and flatten the SSH wavenumber spectra, in agreement with the altimetric spectra. The coherent M2 baroclinic tide is the dominant signal at  ∼ 140&thinsp;km wavelength. At short scales, wavenumber SSH spectra are dominated by incoherent internal tides and internal waves which extend up to 200&thinsp;km in wavelength. These incoherent internal waves impact space scales observed by today's along-track altimetric SSH, and also on the future Surface Water Ocean Topography (SWOT) mission 2-D swath observations, raising the question of altimetric observability of the shorter mesoscale structures in the tropics.</p

The WOCE–era 3–D Pacific Ocean circulation and heat budget

Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Progress In Oceanography 82 (2009): 281-325, doi:10.1016/j.pocean.2009.08.002.To address questions concerning the intensity and spatial structure of the 3–dimensional circulation within the Pacific Ocean and the associated advective and diffusive property flux divergences, data from approximately 3000 high–quality hydrographic stations collected on 40 zonal and meridional cruises have been merged into a physically consistent model. The majority of the stations were occupied as part of the World Ocean Circulation Experiment (WOCE), which took place in the 1990s. These data are supplemented by a few pre–WOCE surveys of similar quality, and time–averaged direct–velocity and historical hydrographic measurements about the equator. An inverse box model formalism is employed to estimate the absolute along–isopycnal velocity field, the magnitude and spatial distribution of the associated diapycnal flow and the corresponding diapycnal advective and diffusive property flux divergences. The resulting large–scale WOCE Pacific circulation can be described as two shallow overturning cells at mid– to low latitudes, one in each hemisphere, and a single deep cell which brings abyssal waters from the Southern Ocean into the Pacific where they upwell across isopycnals and are returned south as deep waters. Upwelling is seen to occur throughout most of the basin with generally larger dianeutral transport and greater mixing occurring at depth. The derived pattern of ocean heat transport divergence is compared to published results based on air–sea flux estimates. The synthesis suggests a strongly east/west oriented pattern of air–sea heat flux with heat loss to the atmosphere throughout most of the western basins, and a gain of heat throughout the tropics extending poleward through the eastern basins. The calculated meridional heat transport agrees well with previous hydrographic estimates. Consistent with many of the climatologies at a variety of latitudes as well, our meridional heat transport estimates tend toward lower values in both hemispheres.This work was funded by National Science Foundation grants OCE–9710102, OCE– 9712209 and OCE–0079383, and also benefited from work on closely related projects funded by NSF grants OCE–0223421 and OCE–0623261, and NOAA grant NA17RJ1223 funded through CICOR. For G.C.J. NASA funding came under Order W–19,314

The human equilibrative nucleoside transporter 1 mediates in vitro cytarabine sensitivity in childhood acute myeloid leukaemia

Cytarabine (ara-C) is the most effective agent for the treatment of acute myeloid leukaemia (AML). Aberrant expression of enzymes involved in the transport/metabolism of ara-C could explain drug resistance. We determined mRNA expression of these factors using quantitative-real-time-PCR in leukemic blasts from children diagnosed with de novo AML. Expression of the inactivating enzyme pyrimidine nucleotidase-I (PN-I) was 1.8-fold lower in FAB-M5 as compared to FAB-M1/2 (P=0.007). In vitro sensitivity to deoxynucleoside analogues was determined using the MTT-assay. Human equilibrative nucleoside transporter-1 (hENT1) mRNA expression and ara-C sensitivity were significantly correlated (rp=−0.46; P=0.001), with three-fold lower hENT1 mRNA levels in resistant patients (P=0.003). hENT1 mRNA expression also seemed to correlate inversely with the LC50 values of cladribine (rp=−0.30; P=0.04), decitabine (rp=−0.29; P=0.04) and gemcitabine (rp=−0.33; P=0.02). Deoxycytidine kinase (dCK) and cytidine deaminase (CDA) mRNA expression seemed to correlate with in vitro sensitivity to gemcitabine (rp=−0.31; P=0.03) and decitabine (rp=0.33; P=0.03), respectively. The dCK/PN-I ratio correlated inversely with LC50 values for gemcitabine (rp=−0.45, P=0.001) and the dCK/CDA ratio seemed to correlate with LC50 values for decitabine (rp=−0.29; 0.04). In conclusion, decreased expression of hENT1, which transports ara-C across the cell membrane, appears to be a major factor in ara-C resistance in childhood AML

The annual cycle of circulation of the southwest subtropical Pacific, analyzed in an ocean GCM

International audienc